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These findings confirmed that slr0236 and sll1545 are, respectively, dispensable and crucial to cell growth in standard photoautotrophic conditions
Membranes were then incubated with one of the following primary antibodies overnight at 4 C: AQP3 (1:1000, Santa Cruz Biotechnology, TX, USA), AQP4 (1:1000, Abcam Biochemicals, Grand Island, NY, USA), claudin-1 (1:1000, Invitrogen Technology, Carlsbad, CA, USA), occludin (1:1000, Invitrogen Technology, Carlsbad, CA, USA), Beclin-1 (1:1000, Cell Signaling Technology, Danvers, MA, USA), Atg5 (1:5000, Abcam Biochemicals, Grand Island, NY, USA), iFABP (1:1000, Santa Cruz Biotechnology, TX, USA), HSP70 (1:1000, Enzo Life Sciences, Inc., NY, USA), LC3 (1:1000, Sigma Aldrich) or -actin (1:5000, Sigma Aldrich)
In a 2-year, 23 h/day, whole-body inhalation study on rats, 0.48 mg/m 3 caused inhibition of plasma and erythrocyte activity, but brain AChE activity was not inhibited and there were no clinical signs
